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| Status |
Public on Mar 21, 2023 |
| Title |
Single cell and spatial transcriptomic profile of whole CLAD and donor control lung tissue |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chronic lung allograft dysfunction (CLAD) is the leading cause of death in lung transplant recipients. CLAD is characterized clinically by a persistent decline in pulmonary function and histologically by the development of airway centered fibrosis known as bronchiolitis obliterans. We performed single-cell sequencing and spatial transcriptomic analysis of explanted tissues from human lung recipients with CLAD and donor control lungs.
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| Overall design |
For the single cell dataset, whole lung tissue segments from four CLAD samples and three donor control samples were used. For the spatial transcriptomic dataset, tissue blocks from three CLAD samples and one donor control sample were used. Donor control tissue was obtained from donor lungs used for transplant that were harvested, perserved, implanted, and then trimmed to fit the recipient chest cavity with excess donor trimming preserved for research. Donor control lung tissue underwent formal pahtology evaluation that confirmed the absence of any significant lung disease.
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| Contributor(s) |
Scott P, Aaditya K, Vaibhav J, Jamie T |
| Citation(s) |
36946463 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R38 HL143612 |
Duke Resident Physician-Scientist Program- NHLBI |
DUKE UNIVERSITY |
Scott M Palmer |
| U01 AI163099 |
Lung Transplant Clinical Trial Network (LT-CTN) |
DUKE UNIVERSITY |
Scott M Palmer |
| R21 TR003037 |
Proof of concept treatment interventions in a rodent model of the rare disease bronchiolitis obliterans |
DUKE UNIVERSITY |
Scott M Palmer |
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| Submission date |
Feb 01, 2023 |
| Last update date |
Mar 23, 2023 |
| Contact name |
Scott Palmer |
| E-mail(s) |
[email protected]
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| Organization name |
Duke University
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| Street address |
2100 Erwin Road
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27705 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA930324 |
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