|
| Status |
Public on Nov 30, 2014 |
| Title |
Prevention and cure of rotavirus infection via TLR5/NLRC4-mediated production of IL-22 and IL-18 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Activators of innate immunity may have potential to combat a broad range of infectious agents. We report that treatment with bacterial flagellin prevented rotavirus (RV) infection in mice and cured chronically RV-infected mice. Protection was independent of adaptive immunity and interferon (IFN, type I and II) and required flagellin receptors Toll-like receptor 5 (TLR5) and NOD-like receptor C4 (NLRC4). Flagellin-induced activation of TLR5 on dendritic cells elicited production of the cytokine interleukin (IL)-22, which induced a protective gene expression program in intestinal epithelial cells. Flagellin also induced NLRC4-dependent production of IL-18 and immediate elimination of RV-infected cells. Administration of IL-22 and IL-18 to mice fully recapitulated the capacity of flagellin to prevent or eliminate RV infection, and thus holds promise as a broad-spectrum antiviral agent.
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| |
|
| Overall design |
Total mRNA from intestinal epithelial cells of Rag1-/- mice treated with PBS, IL-18, IL-22 or IL-22/IL-18 was assayed for RNA sequencing.
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| |
|
| Contributor(s) |
Chassaing B, Zhang B, Gewirtz AT |
| Citation(s) |
25395539 |
| |
| Submission date |
Oct 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Benoit Chassaing |
| E-mail(s) |
[email protected]
|
| Phone |
404 413 3589
|
| Organization name |
Georgia State University
|
| Department |
Biology
|
| Lab |
Gewirtz's lab
|
| Street address |
100 Piedmont Av SE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30303 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA264224 |
| SRA |
SRP049019 |
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