› 1qrr

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE

Released:
DOI: 10.2210/pdb1qrr/pdb
PDB entry 1qrr coloured by chain, front view.
PDB entry 1qrr coloured by chain, side view.
PDB entry 1qrr coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose.
Mulichak AM, Theisen MJ, Essigmann B, Benning C, Garavito RM
Proc Natl Acad Sci U S A 96 13097-102 (1999)
PMID: 10557279

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-sulfoquinovose + H(2)O = UDP-alpha-D-glucose + sulfite
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-sulfoquinovose synthase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O48917 (Residues: 85-477; Coverage: 82%)
Gene names: At4g33030, F26P21.150, SQD1
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand SO4 2 x SO4
Ligand UPG 1 x UPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: I4122
Unit cell:
a: 159.61Å b: 159.61Å c: 98.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.17 0.191
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
6 more

4 mentions without citation

Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose.
Mulichak et al. (1999)
3 more