› 3x1x

X-ray diffraction
1Å resolution

Ras-related protein Rap1B with GppNHp

Released:
DOI: 10.2210/pdb3x1x/pdb
PDB entry 3x1x coloured by chain, front view.
PDB entry 3x1x coloured by chain, side view.
PDB entry 3x1x coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The structure and conformational switching of Rap1B.
Noguchi H, Ikegami T, Nagadoi A, Kamatari YO, Park SY, Tame JR, Unzai S
Biochem Biophys Res Commun 462 46-51 (2015)
PMID: 25935485

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-563144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rap-1b Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.99 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62636 (Residues: 1-167; Coverage: 91%)
Gene name: Rap1b
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand CD 1 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 43.722Å b: 52.46Å c: 60.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.211
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Many Faces of Rap1 GTPase.
Jaśkiewicz et al. (2018)
1 more

1 mention without citation

A saturation-mutagenesis analysis of the interplay between stability and activation in Ras.
Hidalgo et al. (2022)