› 3fsi

X-ray diffraction
1.75Å resolution

Crystal structure of a trypanocidal 4,4'-Bis(imidazolinylamino)diphenylamine bound to DNA

Released:
DOI: 10.2210/pdb3fsi/pdb
PDB entry 3fsi coloured by chain, front view.
PDB entry 3fsi coloured by chain, side view.
PDB entry 3fsi coloured by chain, top view.
Source organism: Moloney murine leukemia virus
Primary publication:
Crystal structure of a trypanocidal 4,4'-bis(imidazolinylamino)diphenylamine bound to DNA.
Glass LS, Nguyen B, Goodwin KD, Dardonville C, Wilson WD, Long EC, Georgiadis MM
Biochemistry 48 5943-52 (2009)
PMID: 19405506

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-507448 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Reverse transcriptase/ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 683-937; Coverage: 15%)
Gene name: gag-pol
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
5'-D(*CP*TP*TP*AP*AP*TP*TP*C)-3' Chain: G
Molecule details ›
Chain: G
Length: 8 nucleotides
Theoretical weight: 2.38 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided
5'-D(P*GP*AP*AP*TP*TP*AP*AP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ACT 5 x ACT
Ligand MWB 1 x MWB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 53.743Å b: 145.592Å c: 46.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 citation in other articles

Survey of the 2009 commercial optical biosensor literature.
Rich et al. (2011)
10 more

8 mentions without citation

Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.
Sheng et al. (2013)
7 more