4p6i

X-ray diffraction
2.3Å resolution

Crystal structure of the Cas1-Cas2 complex from Escherichia coli

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-545739 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cas2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
CRISPR-associated endonuclease Cas1 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 305 amino acids
Theoretical weight: 33.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 94.875Å b: 125.696Å c: 99.306Å
α: 90° β: 102.74° γ: 90°
R-values:
R R work R free
0.226 0.225 0.245
Expression system: Escherichia coli BL21(DE3)