› 4tvp

X-ray diffraction
3.1Å resolution

Crystal Structure of the HIV-1 BG505 SOSIP.664 Env Trimer Ectodomain, Comprising Atomic-Level Definition of Pre-Fusion gp120 and gp41, in Complex with Human Antibodies PGT122 and 35O22

Released:
DOI: 10.2210/pdb4tvp/pdb
PDB entry 4tvp coloured by chain, front view.
PDB entry 4tvp coloured by chain, side view.
PDB entry 4tvp coloured by chain, top view.
Source organisms:
Primary publication:
Structure and immune recognition of trimeric pre-fusion HIV-1 Env.
Pancera M, Zhou T, Druz A, Georgiev IS, Soto C, Gorman J, Huang J, Acharya P, Chuang GY, Ofek G, Stewart-Jones GB, Stuckey J, Bailer RT, Joyce MG, Louder MK, Tumba N, Yang Y, Zhang B, Cohen MS, Haynes BF, Mascola JR, Morris L, Munro JB, Blanchard SC, Mothes W, Connors M, Kwong PD
OpenAccess logo Nature 514 455-61 (2014)
PMID: 25296255

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-558123 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (13 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 481 amino acids
Theoretical weight: 54.06 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 30-505; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S5 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
PGT122 Light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PGT122 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 25.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
35O22 Heavy chain Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 26.17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
35O22 Light chain Chain: E
Molecule details ›
Chain: E
Length: 216 amino acids
Theoretical weight: 23.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 8 x NAG
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P63
Unit cell:
a: 128.89Å b: 128.89Å c: 313.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.214 0.248
Expression system: Homo sapiens

Quick links

Citations

85 review citations

Viral membrane fusion.
Harrison SC. (2015)
84 more

156 mentions without citation

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
155 more