› 5lk4

X-ray diffraction
1.47Å resolution

Structure of the Red Fluorescent Protein mScarlet at pH 7.8

Released:
DOI: 10.2210/pdb5lk4/pdb
PDB entry 5lk4 coloured by chain, front view.
PDB entry 5lk4 coloured by chain, side view.
PDB entry 5lk4 coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
mScarlet: a bright monomeric red fluorescent protein for cellular imaging.
Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW
Nat Methods 14 53-56 (2017)
PMID: 27869816

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.9 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U6Y8 (Residues: 7-221; Coverage: 96%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

4 bound ligands:
Ligand PG4 1 x PG4
Ligand PO4 1 x PO4
Ligand PGE 4 x PGE
Ligand PEG 1 x PEG
1 modified residue:
Ligand NRQ 1 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 84.2Å b: 35.64Å c: 86.01Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.138 0.137 0.167
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.
Greenwald et al. (2018)
22 more

5 mentions without citation

Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins.
Canty et al. (2018)
4 more