› 6h2k

X-ray diffraction
1.9Å resolution

Structure of BlaC from Mycobacterium tuberculosis bound to the trans-enamine adduct of sulbactam.

Released:
DOI: 10.2210/pdb6h2k/pdb
PDB entry 6h2k coloured by chain, front view.
PDB entry 6h2k coloured by chain, side view.
PDB entry 6h2k coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis.
Tassoni R, Blok A, Pannu NS, Ubbink M
OpenAccess logo Biochemistry 58 997-1009 (2019)
PMID: 30632739

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 29.48 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

4 bound ligands:
Ligand TSL 2 x TSL
Ligand PEG 6 x PEG
Ligand GOL 3 x GOL
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P1
Unit cell:
a: 39.71Å b: 41.95Å c: 76.93Å
α: 105.1° β: 89.95° γ: 90.81°
R-values:
R R work R free
0.184 0.182 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography.
Pandey et al. (2021)
7 more

2 mentions without citation

New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis.
Tassoni et al. (2019)
1 more