› 6sof

Electron Microscopy
4.3Å resolution

human insulin receptor ectodomain bound by 4 insulin

Released:
DOI: 10.2210/pdb6sof/pdb
PDB entry 6sof coloured by chain, front view.
PDB entry 6sof coloured by chain, side view.
PDB entry 6sof coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain.
Gutmann T, Schäfer IB, Poojari C, Brankatschk B, Vattulainen I, Strauss M, Coskun Ü
OpenAccess logo J Cell Biol 219 (2020)
PMID: 31727777
Related structures: EMD-10273

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-524965 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Insulin receptor subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 719 amino acids
Theoretical weight: 82.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06213 (Residues: 28-746; Coverage: 53%)
Gene name: INSR
Sequence domains:
Insulin receptor subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 162 amino acids
Theoretical weight: 18.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06213 (Residues: 795-956; Coverage: 12%)
Gene name: INSR
Sequence domains:
Insulin A chain Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-10273
Expression systems:
  • Homo sapiens
  • Saccharomyces cerevisiae

Quick links

Citations

14 review citations

Insulin action at a molecular level - 100 years of progress.
White et al. (2021)
13 more

16 mentions without citation

Understanding insulin and its receptor from their three-dimensional structures.
Lawrence MC. (2021)
15 more