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Summary
The National Institute of Standards and Technology plans to develop a reference material to support reliability in metabolomics measurement. Currently, we have 200 vials of a research-grade testing material (RGTM 10212) containing a "Synthetic fecal metabolite calibrant" solution with 70 compounds representing various chemical classes, including SCFAs, amino acids, phenols, and pyrimidines commonly found in human feces at known biologically relevant concentrations in the gut microbiome.
Description
Research Grade Testing Material (RGTM 10212), Synthetic Fecal Metabolite Calibrant
Metabolomics is an exciting and dynamic field in life sciences that harnesses cutting-edge technologies to identify and characterize small molecules, or metabolites, within cells, tissues, or organisms, collectively known as the metabolome. The wealth of evidence in the literature underscores the crucial role of the gut metabolome in human health, paving the way for its potential as an invaluable tool in clinical applications and diagnostics. Despite the challenges posed by the complexity of samples and analytical techniques, the field is steadily advancing toward translational applications. The collaborative efforts of the NIST microbial metrology program and the metabolomics community are driving progress in this promising area, holding great promise for the future of healthcare and medical research.
The calibrant is designed to evaluate the performance and precision of instruments used in metabolomics analysis of stool or other biological samples. It helps to optimize analytical workflows, data analysis methods, and databases. This applies to techniques such as NMR spectroscopy and gas- and liquid chromatography coupled with mass spectrometry.
This material, with its diverse compound representation and quantification, will promote ground truth, while the human stool reference material ( RM8048) will promote measurement reproducibility. Together, they will ensure the reliability of the analytical workflow, providing a solid foundation for metabolomics research and increasing confidence in the measurement process.