Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division.

Brown KE; Baxter J; Graf D; Merkenschlager M; Fisher AG

doi:10.1016/s1097-2765(00)80311-1

Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division.

Graf D ,

Affiliations

1. Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom.
Authors
Brown KE¹
(1 author)

ORCIDs linked to this article

Molecular Cell, 01 Feb 1999, 3(2):207-217
https://doi.org/10.1016/s1097-2765(00)80311-1 PMID: 10078203

Abstract

We show that several transcriptionally inactive genes localize to centromeric heterochromatin in the nucleus of cycling but not quiescent (noncycling) primary B lymphocytes. In quiescent cells, centromeric repositioning of inactive loci was induced after mitogenic stimulation. A dynamic repositioning of selected genes was also observed in developing T cells. Rag and TdT loci were shown to relocate to centromeric domains following heritable gene silencing in primary CD4+8+ thymocytes, but not in a phenotypically similar cell line in which silencing occurred but was not heritable. Collectively, these data indicate that the spatial organization of genes in cycling and noncycling lymphocytes is different and that locus repositioning may be a feature of heritable gene silencing.

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Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division.

Affiliations

Authors

ORCIDs linked to this article

Abstract

Full text links

References

The role of mel-18, a mammalian Polycomb group gene, during IL-7-dependent proliferation of lymphocyte precursors.

Long-term human B cell lines dependent on interleukin-4 and antibody to CD40.

Segmental distribution of bithorax complex proteins during Drosophila development.

Physical linkage of the murine Hox-b cluster and nerve growth factor receptor on yeast artificial chromosomes.

Exclusion and inclusion of alpha and beta T cell receptor alleles.

DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.

Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection.

Association of transcriptionally silent genes with Ikaros complexes at centromeric heterochromatin.

Buckle, V.J., and Rack, K.A. (1993). Fluorescence in situ hybridization. In Human Genetic Disease Analysis—A Practical Approach, K.E. Davies, ed. (Oxford: IRL Press), pp. 59–82.

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Citations & impact

Impact metrics

Citations of article over time

Article citations

Nucleolus and centromere Tyramide Signal Amplification-Seq reveals variable localization of heterochromatin in different cell types.

Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D.

Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.

Spatial Organization of Chromatin: Transcriptional Control of Adaptive Immune Cell Development.

Epigenetic Factors That Control Pericentric Heterochromatin Organization in Mammals.

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Search life-sciences literature (45,104,206 articles, preprints and more)

Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division.

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Authors

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References

Buckle, V.J., and Rack, K.A. (1993). Fluorescence in situ hybridization. In Human Genetic Disease Analysis—A Practical Approach, K.E. Davies, ed. (Oxford: IRL Press), pp. 59–82.

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