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Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo.

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Affiliations

  • 1. Department of Cell Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA.
      Authors
    • Wu MN 1
    (1 author)

ORCIDs linked to this article

Neuron, 01 Jul 1999, 23(3):593-605
https://doi.org/10.1016/s0896-6273(00)80811-9 PMID: 10433270 

This article has been corrected. See Neuron 2000 Mar;25(3):735.

Abstract 

Biochemical studies suggest that syntaxin 1A participates in multiple protein-protein interactions in the synaptic terminal, but the in vivo significance of these interactions is poorly understood. We used a targeted mutagenesis approach to eliminate specific syntaxin binding interactions and demonstrate that Drosophila syntaxin 1A plays multiple regulatory roles in neurotransmission in vivo. Syntaxin mutations that eliminate ROP/Munc-18 binding display increased neurotransmitter release, suggesting that ROP inhibits neurosecretion through its interaction with syntaxin. Syntaxin mutations that block Ca2+ channel binding also cause an increase in neurotransmitter release, suggesting that syntaxin normally functions in inhibiting Ca2+ channel opening. Additionally, we identify and characterize a syntaxin Ca2+ effector domain, which may spatially organize the Ca2+ channel, cysteine string protein, and synaptotagmin for effective excitation-secretion coupling in the presynaptic terminal.

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Read article at publisher's site: https://doi.org/10.1016/s0896-6273(00)80811-9

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Funding 

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Howard Hughes Medical Institute

    NIGMS NIH HHS (1)

    National Institute of Mental Health

      National Institutes of Health (1)