Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

Kaesler S; Lüscher B; Rüther U

doi:10.1515/bc.2000.070

Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

Kaesler S ¹,

Lüscher B ,

Rüther U

Affiliations

1. Institut für Molekularbiologie, Medizinische Hochschule Hannover, Germany.
Authors
Kaesler S¹
(1 author)

ORCIDs linked to this article

Lüscher B | 0000-0002-9622-8709

Biological Chemistry, 01 Jul 2000, 381(7):545-551
https://doi.org/10.1515/bc.2000.070 PMID: 10987360

Abstract

The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence for proteolytic processing or for alteration in the subcellular distribution of GLI1 was obtained. The responsive PKA site (aa333-336) was localized to the second zinc finger of GLI1. Mutation of Ser336 revealed that PKA could also stimulate GLI1 transcriptional activity. Thus, our data demonstrate both negative and positive regulation of human GLI1 by PKA.

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References

Articles referenced by this article (50)

Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins.
Alexandre C, Jacinto A, Ingham PW
Genes Dev, (16):2003-2013 1996
MED: 8769644
Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development.
Buscher D, Bosse B, Heymer J, Ruther U
Mech Dev, (2):175-182 1997
MED: 9152009
Regulation of transcription factors c-Myc, Max, and c-Myb by casein kinase II.
Bousset K, Oelgeschlager MH, Henriksson M, Schreek S, Burkhardt H, Litchfield DW, Luscher-Firzlaff JM, Luscher B
Cell Mol Biol Res, (5-6):501-511 1994
MED: 7735324
Protein kinase A directly regulates the activity and proteolysis of cubitus interruptus.
Chen Y, Gallaher N, Goodman RH, Smolik SM
Proc Natl Acad Sci U S A, (5):2349-2354 1998
MED: 9482888
Nuclear trafficking of Cubitus interruptus in the transcriptional regulation of Hedgehog target gene expression.
Chen CH, von Kessler DP, Park W, Wang B, Ma Y, Beachy PA
Cell, (3):305-316 1999
MED: 10458606
Spatial regulation of a zebrafish patched homologue reflects the roles of sonic hedgehog and protein kinase A in neural tube and somite patterning.
Concordet JP, Lewis KE, Moore JW, Goodrich LV, Johnson RL, Scott MP, Ingham PW
Development, (9):2835-2846 1996
MED: 8787757
Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours.
Dahmane N, Lee J, Robins P, Heller P, Ruiz i Altaba A
Nature, (6653):876-881 1997
MED: 9349822
Sonic Hedgehog-induced activation of the Gli1 promoter is mediated by GLI3.
Dai P, Akimaru H, Tanaka Y, Maekawa T, Nakafuku M, Ishii S
J Biol Chem, (12):8143-8152 1999
MED: 10075717
A protein kinase inhibitor gene reduces both basal and multihormone-stimulated prolactin gene transcription.
Day RN, Walder JA, Maurer RA
J Biol Chem, (1):431-436 1989
MED: 2535842
Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice.
Ding Q, Motoyama J, Gasca S, Mo R, Sasaki H, Rossant J, Hui CC
Development, (14):2533-2543 1998
MED: 9636069

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Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

Affiliations

Authors

ORCIDs linked to this article

Abstract

Full text links

References

Transcriptional activation of hedgehog target genes in Drosophila is mediated directly by the cubitus interruptus protein, a member of the GLI family of zinc finger DNA-binding proteins.

Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development.

Regulation of transcription factors c-Myc, Max, and c-Myb by casein kinase II.

Protein kinase A directly regulates the activity and proteolysis of cubitus interruptus.

Nuclear trafficking of Cubitus interruptus in the transcriptional regulation of Hedgehog target gene expression.

Spatial regulation of a zebrafish patched homologue reflects the roles of sonic hedgehog and protein kinase A in neural tube and somite patterning.

Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours.

Sonic Hedgehog-induced activation of the Gli1 promoter is mediated by GLI3.

A protein kinase inhibitor gene reduces both basal and multihormone-stimulated prolactin gene transcription.

Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice.

Citations & impact

Impact metrics

Citations of article over time

Article citations

Novel Approaches in Non-Melanoma Skin Cancers-A Focus on Hedgehog Pathway in Basal Cell Carcinoma (BCC).

Hedgehog Signaling and Truncated GLI1 in Cancer.

Tumour suppressor 15-hydroxyprostaglandin dehydrogenase induces differentiation in colon cancer via GLI1 inhibition.

Differential phosphorylation determines the repressor and activator potencies of GLI1 proteins and their efficiency in modulating the HPV life cycle.

Potential use of CXCL12/CXCR4 and sonic hedgehog pathways as therapeutic targets in medulloblastoma.

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Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

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