Transforming properties of a Q18-->E mutation of the microtubule regulator Op18.

Misek DE; Chang CL; Kuick R; Hinderer R; Giordano TJ; Beer DG; Hanash SM

doi:10.1016/s1535-6108(02)00124-1

Transforming properties of a Q18-->E mutation of the microtubule regulator Op18.

Affiliations

1. Department of Pediatrics, University of Michigan, Ann Arbor, MI 48109, USA.
Authors
Misek DE¹
(1 author)

ORCIDs linked to this article

Giordano TJ | 0000-0003-0641-8873

Cancer Cell, 01 Sep 2002, 2(3):217-228
https://doi.org/10.1016/s1535-6108(02)00124-1 PMID: 12242154

Abstract

We have identified a somatic mutation in Op18 in a human esophageal adenocarcinoma. The mutant form of Op18 (M-Op18) was cloned and sequenced, revealing a substitution of a G for C at nucleotide 155, which results in a Q18-->E substitution in the protein. M-Op18 cDNA was expressed in NIH/3T3 cells, which resulted in foci formation and tumor growth in immunodeficient mice. Cell cycle analysis of M-Op18-expressing cells revealed a doubling in the percentage of cells in G2/M relative to cells overexpressing wild-type Op18, a decrease in M-Op18-specific phosphorylation, and alterations in tubulin ultrastructure in M-Op18-expressing cells. These results suggest that the somatic mutation identified in Op18 has profound effects on cell homeostasis that may lead to tumorigenicity.

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Funding

Funders who supported this work.

NCI NIH HHS (2)

Grant ID: CA71606
14 publications
Grant ID: CA84953
7 publications

National Cancer Institute (1)

Grant ID: CA84953
2 publications

National Institutes of Health (1)

Grant ID: CA71606
1 publication

Search life-sciences literature (45,103,589 articles, preprints and more)

Transforming properties of a Q18-->E mutation of the microtubule regulator Op18.

Affiliations

Authors

ORCIDs linked to this article

Abstract

Full text links

References

Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.

Multiple phosphorylation of stathmin. Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2.

Quantitative analysis of the expression and regulation of an activation-regulated phosphoprotein (oncoprotein 18) in normal and neoplastic cells.

Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38.

Cdc2 regulatory factors.

Human peripheral lymphocyte growth regulation and response to phorbol esters is linked to synthesis and phosphorylation of the cytosolic protein, prosolin.

Molecular characterization of human stathmin expressed in Escherichia coli: site-directed mutagenesis of two phosphorylatable serines (Ser-25 and Ser-63).

Overexpression of stathmin in breast carcinomas points out to highly proliferative tumours.

Chromosome 1 aneusomy with 1p36 under-representation is related to histologic grade, DNA aneuploidy, high c-erb B-2 and loss of bcl-2 expression in ductal breast carcinoma.

A gene that encodes for a leukemia-associated phosphoprotein (p18) maps to chromosome bands 1p35-36.1.

Citations & impact

Impact metrics

Citations of article over time

Article citations

The microRNA-210-Stathmin1 Axis Decreases Cell Stiffness to Facilitate the Invasiveness of Colorectal Cancer Stem Cells.

miR-34a Regulates Expression of the Stathmin-1 Oncoprotein and Prostate Cancer Progression.

RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis.

Merkel cell polyomavirus small T antigen mediates microtubule destabilization to promote cell motility and migration.

Stathmin 1 in normal and malignant hematopoiesis.

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Funding

NCI NIH HHS (2)

National Cancer Institute (1)

National Institutes of Health (1)

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Search life-sciences literature (45,103,589 articles, preprints and more)

Transforming properties of a Q18-->E mutation of the microtubule regulator Op18.

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Authors

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Funding

NCI NIH HHS (2)﻿

National Cancer Institute (1)﻿

National Institutes of Health (1)﻿

Partnerships & funding

NCI NIH HHS (2)

National Cancer Institute (1)

National Institutes of Health (1)