Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C.

Author information

Affiliations

  • 1. Department of Chemical Engineering and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
      Authors
    • Graff CP 1
    (1 author)

Protein Engineering, Design & Selection : PEDS, 28 Apr 2004, 17(4):293-304
https://doi.org/10.1093/protein/gzh038 PMID: 15115853 

Abstract 

An scFv has been engineered to bind carcinoembryonic antigen (CEA) with a dissociation half-time >4 days at 37 degrees C. Two mutations responsible for this affinity increase were isolated by screening yeast surface-displayed mutant libraries by flow cytometry. Soluble expression of the mutant scFv in a yeast secretion system was increased 100-fold by screening mutant libraries for improved yeast surface display level. This scFv will be useful as a limiting case for evaluating the significance of affinity in tumor targeting to non-internalizing antigens.

Full text links 

Read article at publisher's site: https://doi.org/10.1093/protein/gzh038

Read article for free, from open access legal sources, via Unpaywall: https://academic.oup.com/peds/article-pdf/17/4/293/4694368/gzh038.pdfUnpaywall

Citations & impact 

Impact metrics

94
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/4211647
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/4211647

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1093/protein/gzh038

Supporting
Mentioning
Contrasting
3
95
2

Article citations

Go to all (94) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.