Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Retinal biosynthesis in Eubacteria: in vitro characterization of a novel carotenoid oxygenase from Synechocystis sp. PCC 6803.

Author information

Affiliations

  • 1. Albert-Ludwigs University of Freiburg, Institut for Biology II, Cellbiology, Schaenzlestr. 1, D-79104 Freiburg, Germany.
      Authors
    • Ruch S 1
    (1 author)

ORCIDs linked to this article

Molecular Microbiology, 01 Feb 2005, 55(4):1015-1024
https://doi.org/10.1111/j.1365-2958.2004.04460.x PMID: 15686550 

Abstract 

Retinal and its derivatives represent essential compounds in many biological systems. In animals, they are synthesized through a symmetrical cleavage of beta-carotene catalysed by a monooxygenase. Here, we demonstrate that the open reading frame sll1541 from the cyanobacterium Synechocystis sp. PCC 6803 encodes the first eubacterial, retinal synthesizing enzyme (Diox1) thus far reported. In contrast to enzymes from animals, Diox1 converts beta-apo-carotenals instead of beta-carotene into retinal in vitro. The identity of the enzymatic product was proven by HPLC, GC-MS and in a biological test. Investigations, of the stereospecifity showed that Diox1 cleaved only the all-trans form of beta-apo-8'-carotenal, yielding all-trans-retinal. However, Diox1 exhibited wide substrate specificity with respect to chain-lengths and functional end-groups. Although with divergent Km and Vmax values, the enzyme converted beta-apo-carotenals, (3R)-3-OH-beta-apo-carotenals as well as apo-lycopenals into retinal, (3R)-3-hydroxy-retinal and acycloretinal respectively. In addition, the alcohols of these substrates were cleaved to yield the corresponding retinal derivatives.

Full text links 

Read article at publisher's site: https://doi.org/10.1111/j.1365-2958.2004.04460.x

References 

Articles referenced by this article (41)

Show 10 more references (10 of 41)

Citations & impact 

Impact metrics

74
Citations
Jump to Citations
11
Data citations
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/3657566
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/3657566

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1111/j.1365-2958.2004.04460.x

Supporting
Mentioning
Contrasting
12
131
0

Article citations

Go to all (74) article citations

Other citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.