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High-throughput mapping of a dynamic signaling network in mammalian cells.

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Affiliations

  • 1. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5.
      Authors
    • Barrios-Rodiles M 1
    (1 author)

ORCIDs linked to this article

Science, 01 Mar 2005, 307(5715):1621-1625
https://doi.org/10.1126/science.1105776 PMID: 15761153 

Abstract 

Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions.

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Read article at publisher's site: https://doi.org/10.1126/science.1105776

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Funding 

Funders who supported this work.

NIGMS NIH HHS (1)