Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties.

Kaelin WG Jr; Krek W; Sellers WR; DeCaprio JA; Ajchenbaum F; Fuchs CS; Chittenden T; Li Y; Farnham PJ; Blanar MA

doi:10.1016/0092-8674(92)90108-o

Abstract

An expression vector was modified to permit the rapid synthesis of purified, 32P-labeled, glutathione S-transferase (GST)-retinoblastoma (RB) fusion proteins. The products were used to screen lambda gt11 expression libraries, from which we cloned a cDNA encoding a polypeptide (RBAP-1) capable of binding directly to a putative functional domain (the pocket) of the retinoblastoma gene product (RB). The RB "pocket" is known to bind, directly or indirectly, to the cellular transcription factor, E2F, implicated in cell growth control. We have found that RBAP-1 copurifies with E2F, interacts specifically with the adenovirus E4 ORF 6/7 protein, binds specifically and directly to a known E2F DNA recognition sequence, and contains a functional tranasactivation domain. Therefore, RBAP-1 is a species of E2F and can bind specifically to the RB pocket.

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References

Articles referenced by this article (73)

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Data

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This data has been provided by curated databases and other sources that have cited the article.

Diseases in OMIM

E2F TRANSCRIPTION FACTOR 1; E2F1(OMIM - 189971)

Proteins in UniProt

Transcription factor E2F1(UniProt - Q01094)

GlyGen is an international glycobioinformatics knowledgebase funded by The National Institutes of Health to facilitate glycoscience research by integrating diverse kinds of information, including glycomics, genomics, proteomics (and glycoproteomics), cell biology, developmental biology and biochemistry.

https://www.glygen.org/publication/MED/1638635

Nucleotide sequences in ENA (2)

pGEX-2TK cloning vector, complete sequence.(ENA - U13851)
unidentified cloning vector glutathione S-transferase(ENA - AAA57092)

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Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties.

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Abstract

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References

Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent.

Title not supplied

Phosphorylation-dependent activation of the adenovirus-inducible E2F transcription factor in a cell-free system

The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F.

Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas.

Adenovirus E1a prevents the retinoblastoma gene product from complexing with a cellular transcription factor.

Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells.

Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo.

Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos.

Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Smart citations by scite.ai
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1016/0092-8674(92)90108-o

Article citations

Direct Regulation of DNA Repair by E2F and RB in Mammals and Plants: Core Function or Convergent Evolution?

Regulation of E2F1 activity via PKA-mediated phosphorylations.

Validating the inhibitory effects of d- and l-serine on the enzyme activity of d-3-phosphoglycerate dehydrogenases that are purified from Pseudomonas aeruginosa, Escherichia coli and human colon.

Effector Gene xopAE of Xanthomonas euvesicatoria 85-10 Is Part of an Operon and Encodes an E3 Ubiquitin Ligase.

Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes.

Data

Data that cites the article

Diseases in OMIM

Proteins in UniProt

Nucleotide sequences in ENA (2)

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Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties.

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Phosphorylation-dependent activation of the adenovirus-inducible E2F transcription factor in a cell-free system

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