Acute L-glutamine deprivation compromises VEGF-a upregulation in A549/8 human carcinoma cells.

Drogat B; Bouchecareilh M; North S; Petibois C; Déléris G; Chevet E; Bikfalvi A; Moenner M

doi:10.1002/jcp.21044

Acute L-glutamine deprivation compromises VEGF-a upregulation in A549/8 human carcinoma cells.

Affiliations

1. INSERM, E0113, Université Bordeaux 1, Talence, France.
Authors
Drogat B¹
(1 author)

ORCIDs linked to this article

Journal of Cellular Physiology, 01 Aug 2007, 212(2):463-472
https://doi.org/10.1002/jcp.21044 PMID: 17348020

Abstract

Tumor ischemia participates in angiogenesis and cancer progression through cellular responses to hypoxia and nutrient deprivation. However, the contribution of amino acids limitation to this process remains poorly understood. Using serum-free cell culture conditions, we tested the impact of L-glutamine deprivation on metabolic and angiogenic responses in A549/8 carcinoma cells. In these cells, lowering glutamine concentration modified the cell cycle distribution and significantly induced apoptosis/necrosis. Although glutamine deprivation led to a HIF-independent increase in VEGF-A mRNA, the corresponding protein level remained low and correlated with the inhibition of protein synthesis and activation of the GCN2/eIF2alpha pathway. Limitation of glutamine availability also hampers hypoxia- and hypoglycemia-induced VEGF-A protein upregulation. Thus, glutamine deprivation may have no direct effect on VEGF-dependent angiogenesis, compared to hypoxia or to glucose deprivation, and may instead be detrimental to cancer progression by antagonizing ischemia-induced stresses.

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Read article at publisher's site: https://doi.org/10.1002/jcp.21044

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Proteins in UniProt (Showing 5 of 9)

Platelet-derived growth factor (PDGF) family profile domain-containing protein(UniProt - A0A0Y0J542)
Vascular endothelial growth factor(UniProt - Q9UNS8)
Vascular permeability factor(UniProt - A2A2V4)
Platelet-derived growth factor (PDGF) family profile domain-containing protein(UniProt - Q59FH5)
Platelet-derived growth factor (PDGF) family profile domain-containing protein(UniProt - Q6WZM0)

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Acute L-glutamine deprivation compromises VEGF-a upregulation in A549/8 human carcinoma cells.

Affiliations

Authors

ORCIDs linked to this article

Abstract

Full text links

References

Glutamine deprivation induces the expression of GADD45 and GADD153 primarily by mRNA stabilization.

Response of VEGF expression to amino acid deprivation and inducers of endoplasmic reticulum stress.

Serum-free cell culture: a unifying approach.

[1-(13)C]glucose metabolism in the tumoral and nontumoral cerebral tissue of a glioma-bearing rat.

Ultraviolet B and H2O2 are potent inducers of vascular endothelial growth factor expression in cultured keratinocytes.

Mammalian unfolded protein response inhibits cyclin D1 translation and cell-cycle progression.

Title not supplied

Molecular mechanisms of glutamine action.

Oncogenic alterations of metabolism.

Electrical coupling and plasticity of the mitochondrial network.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Article citations

Multifaceted role of GCN2 in tumor adaptation and therapeutic targeting.

Reprogramming of Glutamine Amino Acid Transporters Expression and Prognostic Significance in Hepatocellular Carcinoma.

Tumour microenvironment in pheochromocytoma and paraganglioma.

ERN1 dependent impact of glucose and glutamine deprivations on PBX3, PBXIP1, PAX6, MEIS1, and MEIS2 genes expression in U87 glioma cells.

Cancer Cell Metabolism in Hypoxia: Role of HIF-1 as Key Regulator and Therapeutic Target.

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Acute L-glutamine deprivation compromises VEGF-a upregulation in A549/8 human carcinoma cells.

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