Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis.

Author information

Affiliations

  • 1. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
      Authors
    • Hold GL 1
    (1 author)

ORCIDs linked to this article

FEMS Microbiology Ecology, 01 Jan 2002, 39(1):33-39
https://doi.org/10.1111/j.1574-6941.2002.tb00904.x PMID: 19709182 

Abstract 

Abstract The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25% of sequences were closely related (>97%) to current species type strains, and 28% were less than 97% related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the first molecular information on the microbial diversity present in human colonic samples.

Full text links 

Read article at publisher's site: https://doi.org/10.1111/j.1574-6941.2002.tb00904.x

Read article for free, from open access legal sources, via Unpaywall: https://academic.oup.com/femsec/article-pdf/39/1/33/18089610/39-1-33.pdfUnpaywall

References 

Articles referenced by this article (38)

Show 10 more references (10 of 38)

Citations & impact 

Impact metrics

207
Citations
Jump to Citations
10
Data citations
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/3342749
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/3342749

Article citations

Go to all (207) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.