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Gene and genome construction in yeast.

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Affiliations

  • 1. The J. Craig Venter Institute, San Diego, California, USA.
      Authors
    • Gibson DG 1
    (1 author)

Current Protocols in Molecular Biology, 01 Apr 2011, Chapter 3:Unit3.22
https://doi.org/10.1002/0471142727.mb0322s94 PMID: 21472698 

Abstract 

The yeast Saccharomyces cerevisiae has the capacity to take up and assemble dozens of different overlapping DNA molecules in one transformation event. These DNA molecules can be single-stranded oligonucleotides, to produce gene-sized fragments, or double-stranded DNA fragments, to produce molecules up to hundreds of kilobases in length, including complete bacterial genomes. This unit presents protocols for designing the DNA molecules to be assembled, transforming them into yeast, and confirming their assembly.

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Read article at publisher's site: https://doi.org/10.1002/0471142727.mb0322s94

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