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Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy.

Author information

Affiliations

  • 1. Laboratory for General Biochemistry & Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
      Authors
    • Martens TF 1
    (1 author)

ORCIDs linked to this article

Nanomedicine (London, England), 26 Feb 2013, 8(12):1955-1968
https://doi.org/10.2217/nnm.12.202 PMID: 23438206 

Abstract 

Aim

To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body.

Materials & methods

Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA.

Results

The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility.

Conclusion

An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration.

Full text links 

Read article at publisher's site: https://doi.org/10.2217/nnm.12.202

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