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Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping.

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Affiliations

  • 1. Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA.
      Authors
    • Waggoner JJ 1
    (1 author)

ORCIDs linked to this article

Journal of Clinical Microbiology, 31 Jul 2013, 51(10):3418-3420
https://doi.org/10.1128/jcm.01359-13 PMID: 23903549 PMCID: PMC3811623

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A comment on this article appears in "Inconclusive reverse transcription-PCR assay comparison for dengue virus detection and serotyping." J Clin Microbiol. 2014 May;52(5):1800. A comment on this article appears in "Reply to "Inconclusive reverse transcription-PCR assay comparison for dengue virus detection and serotyping"." J Clin Microbiol. 2014 May;52(5):1801-2.

Abstract 

Dengue virus (DENV) is the agent of the most common vector-borne disease worldwide. Using 199 clinical samples collected from Nicaragua and Sri Lanka, a laboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.

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Time: 2024/11/20 08:44:47

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Read article at publisher's site: https://doi.org/10.1128/jcm.01359-13

Read article for free, from open access legal sources, via Unpaywall: https://jcm.asm.org/content/jcm/51/10/3418.full.pdfUnpaywall

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Funding 

Funders who supported this work.

CCR NIH HHS (1)

FIC NIH HHS (1)

NIAID NIH HHS (6)