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Recent applications of fluorescence correlation spectroscopy in live systems.

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Affiliations

  • 1. Departments of Biological Sciences and Chemistry and Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore.
      Authors
    • Macháň R 1
    • Wohland T 1
    (2 authors)

ORCIDs linked to this article

FEBS Letters, 12 Apr 2014, 588(19):3571-3584
https://doi.org/10.1016/j.febslet.2014.03.056 PMID: 24726724 

Review

Abstract 

Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and has helped address many questions in the life sciences. It provides important advantages compared to other fluorescence and biophysical methods. Its single molecule sensitivity allows measuring proteins within biological samples at physiological concentrations without the need of overexpression. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions even in live organisms. And its reliance on simple fluorescence intensity and its fluctuations makes it widely applicable. In this review we focus on applications of FCS in live samples, with an emphasis on work in the last 5 years, in the hope to provide an overview of the present capabilities of FCS to address biologically relevant questions.

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Funding 

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Singapore Ministry of Education (1)