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Induction of HIV-1 gag specific immune responses by cationic micelles mediated delivery of gag mRNA.

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Affiliations

  • 1. Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education , West China School of Pharmacy, Sichuan University , Chengdu , People's Republic of China.
      Authors
    • Zhao M 1
    • Li M 1
    • Zhang Z 1
    • Gong T 1
    • Sun X 1
    (5 authors)

ORCIDs linked to this article

Drug Delivery, 29 May 2015, 23(7):2596-2607
https://doi.org/10.3109/10717544.2015.1038856 PMID: 26024387 

Abstract 

In recent years, mRNA-based vaccines have emerged to be a great alternative to DNA-based vaccines due to the safety of not inserting into host genome. However, mRNA molecules are single-stranded nucleic acids that are vulnerable under RNase existing in human skin and tissues. In this study, a self-assembled cationic nanomicelles based on polyethyleneimine-stearic acid (PSA) copolymer were developed to delivery HIV-1 gag encoding mRNA to dendritic cells and BALB/c mice. We evaluated the transfection efficiency and cell uptake efficiency of naked EGFP mRNA, PSA, PEI-2k and PEI-25k nanoparticles format on DC2.4 cell lines. Immune responses after sub-cutaneous administration of gag mRNA to BALB/c mice were notably induced by PSA as compared with naked gag mRNA. We found the PSA/mRNA nanomicelles were potent systems that can effectively deliver mRNA and induce antigen-specific immune response, stimulating various new vaccine strategies using mRNA.

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Read article at publisher's site: https://doi.org/10.3109/10717544.2015.1038856

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