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A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.

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Affiliations

  • 1. Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil.
      Authors
    • Fonseca EL 1
    • Ramos ND 1
    • Andrade BG 1
    • Marin MF 1
    • Vicente AC 1
    (5 authors)
  • 2. Laboratório de Microbiologia Ambiental, Seção de Meio Ambiente, Instituto Evandro Chagas, Belém, Pará, Brazil.
      Authors
    • Morais LL 2
    (1 author)

ORCIDs linked to this article

Diagnostic Microbiology and Infectious Disease, 17 Jan 2017, 87(4):315-317
https://doi.org/10.1016/j.diagmicrobio.2017.01.005 PMID: 28139276 

Abstract 

Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.

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Read article at publisher's site: https://doi.org/10.1016/j.diagmicrobio.2017.01.005

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