The Molecular Revolution in Cutaneous Biology: Era of Next-Generation Sequencing.

Sarig O; Sprecher E

doi:10.1016/j.jid.2016.02.818

The Molecular Revolution in Cutaneous Biology: Era of Next-Generation Sequencing.

Sarig O ¹,

Sprecher E ²

Affiliations

1. Department of Dermatology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
Authors
Sarig O¹
(1 author)
2. Department of Dermatology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel; Department of Human Molecular Genetics & Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Authors
Sprecher E²
(1 author)

The Journal of Investigative Dermatology, 01 May 2017, 137(5):e79-e82
https://doi.org/10.1016/j.jid.2016.02.818 PMID: 28411851

Review

Abstract

Like any true conceptual revolution, next-generation sequencing (NGS) has not only radically changed research and clinical practice, it has also modified scientific culture. With the possibility to investigate DNA contents of any organism and in any context, including in somatic disorders or in tissues carrying complex microbial populations, it initially seemed as if the genetic underpinning of any biological phenomenon could now be deciphered in an almost streamlined fashion. However, over the past recent years, we have once again come to understand that there is no such a thing as great opportunities without great challenges. The steadily expanding use of NGS and related applications is now facing biologists and physicians with novel technological obstacles, analytical hurdles and increasingly pressing ethical questions.

Full text links

Read article at publisher's site: https://doi.org/10.1016/j.jid.2016.02.818

Read article for free, from open access legal sources, via Unpaywall: http://www.jidonline.org/article/S0022202X16327804/pdf

References

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Search life-sciences literature (45,103,589 articles, preprints and more)

The Molecular Revolution in Cutaneous Biology: Era of Next-Generation Sequencing.

Affiliations

Authors

Authors

Abstract

Full text links

References

Confirming Variants in Next-Generation Sequencing Panel Testing by Sanger Sequencing.

Comparison of commercially available target enrichment methods for next-generation sequencing

Modeling genomic regulatory networks with big data.

Rare-disease genetics in the era of next-generation sequencing: discovery to translation.

Dominant De Novo Mutations in GJA1 Cause Erythrokeratodermia Variabilis et Progressiva, without Features of Oculodentodigital Dysplasia.

Somatic mosaicism: implications for disease and transmission genetics.

Noninvasive Prenatal Screening for Genetic Diseases Using Massively Parallel Sequencing of Maternal Plasma DNA.

Uncovering the roles of rare variants in common disease through whole-genome sequencing.

Filaggrin-stratified transcriptomic analysis of pediatric skin identifies mechanistic pathways in patients with atopic dermatitis.

Homozygosity mapping and whole-exome sequencing to detect SLC45A2 and G6PC3 mutations in a single patient with oculocutaneous albinism and neutropenia.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Article citations

Targeting SERCA2 in organotypic epidermis reveals MEK inhibition as a therapeutic strategy for Darier disease.

Gene expression profile analysis on different stages of hypertrophic scarring in a rabbit ear model.

Molecular Genetics of Keratinization Disorders - What's New About Ichthyosis.

Autosomal recessive congenital ichthyosis: Genomic landscape and phenotypic spectrum in a cohort of 125 consanguineous families.

Coinheritance of biallelic SLURP1 and SLC39A4 mutations cause a severe genodermatosis with skin peeling and hair loss all over the body.

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The Molecular Revolution in Cutaneous Biology: Era of Next-Generation Sequencing.

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Authors

Authors

Abstract

Full text links

References

Comparison of commercially available target enrichment methods for next-generation sequencing

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