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Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.

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Affiliations

  • 1. New England BioLabs, Beverly, MA 01915.
      Authors
    • di Guan C 1
    (1 author)

Gene, 01 Jul 1988, 67(1):21-30
https://doi.org/10.1016/0378-1119(88)90004-2 PMID: 2843437 

Abstract 

Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins. The peptides are fused to the C terminus of maltose-binding protein (MBP), which allows them to be purified by the MBP's affinity to cross-linked amylose (starch). The fusion protein can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.

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Read article at publisher's site: https://doi.org/10.1016/0378-1119(88)90004-2

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