The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.

Blom van Assendelft G; Hanscombe O; Grosveld F; Greaves DR

doi:10.1016/0092-8674(89)90630-2

The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.

Blom van Assendelft G ¹,

Hanscombe O ,

Grosveld F ,

Greaves DR

Affiliations

1. Laboratory of Gene Structure and Expression, National Institute for Medical Research, London, England.
Authors
Blom van Assendelft G¹
(1 author)

ORCIDs linked to this article

Greaves DR | 0000-0003-2856-9410

Cell, 01 Mar 1989, 56(6):969-977
https://doi.org/10.1016/0092-8674(89)90630-2 PMID: 2924354

Abstract

We have introduced a human beta-globin minilocus, containing the recently described dominant control region (DCR), the beta-globin or Thy-1 gene, and a thymidine kinase (tk)-neoR gene into erythroid and non-erythroid cells. Analysis of the transcription levels of the genes shows that the DCR directs high levels of human beta-globin, Thy-1 and tk-neo expression independent of integration sites in an erythroid-specific manner. The presence of the DNAasel hypersensitive sites at the 5' end of the locus is required for this effect on the homologous and heterologous gene. An analysis of the DCR chromatin in transfected mouse erythroleukemic cells suggests that the formation of the hypersensitive sites in this region precedes beta-globin gene expression.

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Diseases in OMIM

HEMOGLOBIN--BETA LOCUS; HBB(OMIM - 141900)

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The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.

Affiliations

Authors

ORCIDs linked to this article

Abstract

Full text links

References

Molecular characterization of a beta zero-thalassemia resulting from a 1.4 kilobase deletion.

β-globin gene promoter generates 5′ truncated transcripts in the embryonic foetal erythroid environment

The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.

The beta-globin gene on the Chinese delta beta-thalassemia chromosome carries a promoter mutation.

Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.

Rapid reprogramming of globin gene expression in transient heterokaryons.

Two 3′ sequences direct erythroid specific expression of human β-globin genes in transgenic mice

DNA methylation and the regulation of globin gene expression.

Developmental regulation of beta-globin gene switching.

The molecular genetics of human hemoglobin

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Smart citations by scite.ai
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1016/0092-8674(89)90630-2

Article citations

Tales from topographic oceans: topologically associated domains and cancer.

Chromatin priming elements establish immunological memory in T cells without activating transcription: T cell memory is maintained by DNA elements which stably prime inducible genes without activating steady state transcription.

Genome-wide mapping of autonomous promoter activity in human cells.

Treating hemoglobinopathies using gene-correction approaches: promises and challenges.

Enhancing our understanding of enhancers in T-helper cells.

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Search life-sciences literature (45,104,206 articles, preprints and more)

The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.

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Authors

ORCIDs linked to this article

Abstract

Full text links

References

β-globin gene promoter generates 5′ truncated transcripts in the embryonic foetal erythroid environment

Two 3′ sequences direct erythroid specific expression of human β-globin genes in transgenic mice

The molecular genetics of human hemoglobin

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Citations of article over time

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