Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Immunofluorescence Study of Endogenous YAP in Mammalian Cells.

Author information

Affiliations

  • 1. University of Edinburgh Centre for Inflammation Research, Queen's Medical Research Institute, Edinburgh, UK.
      Authors
    • Rausch V 1
    • Hansen CG 1
    (2 authors)

ORCIDs linked to this article

Methods in Molecular Biology (Clifton, N.J.), 01 Jan 2019, 1893:97-106
https://doi.org/10.1007/978-1-4939-8910-2_8 PMID: 30565128 

Abstract 

Immunocytochemistry enables determination of cellular localization and relative abundance of proteins. This protocol describes a rapid and cost-effective approach to study the cellular localization of YAP (and TAZ), the transcriptional co activators of the Hippo pathway, in mammalian cells. Cells are seeded onto coated cover slips, cultivated and treated as required. Subsequently, they are chemically fixed, and cellular proteins are fluorescently labeled by means of specific antibodies. Multiplexing antibodies enables ascertaining the subcellular localization of YAP and TAZ and thereby also the activation state of the Hippo pathway in various cell types.

Full text links 

Read article at publisher's site: https://doi.org/10.1007/978-1-4939-8910-2_8

Citations & impact 

Impact metrics

5
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/52978380
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/52978380

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1007/978-1-4939-8910-2_8

Supporting
Mentioning
Contrasting
0
12
0

Article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Wellcome Trust