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A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses.

Author information

Affiliations

  • 1. Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
      Authors
    • Takada K 1
    • Takasaki S 1
    • Sakai-Tagawa Y 1
    • Lopes TJS 1, 3
    • Yamada S 1
    • Watanabe T 1
    • Imai M 1
    • Kawaoka Y 1, 3
    (8 authors)
  • 2. Yokohama City Institute of Public Health, Kanagawa, Japan.
      Authors
    • Kawakami C 2
    • Shimizu K 2
    (2 authors)
  • 3. Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
      Authors
    • Fan S 3
    • Chiba S 3
    • Zhong G 3
    • Gu C 3
    • Lopes TJS 1, 3
    • Kawaoka Y 1, 3
    (6 authors)
  • 4. Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
      Authors
    • Dutta J 4
    • Khan Z 4
    • Kriti D 4
    • van Bakel H 4
    (4 authors)

ORCIDs linked to this article

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Nature Microbiology, 29 Apr 2019, 4(8):1268-1273
https://doi.org/10.1038/s41564-019-0433-6 PMID: 31036910 

Abstract 

Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.

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Read article at publisher's site: https://doi.org/10.1038/s41564-019-0433-6

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Funding 

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NIH HHS (1)