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Heterogeneity of acid beta-galactosidase from rabbit kidney.

The International Journal of Biochemistry, 01 Jan 1987, 19(8):685-691
https://doi.org/10.1016/0020-711x(87)90081-4 PMID: 3114021 

Abstract 

1. Rabbit kidney acid beta-galactosidase can be resolved into three peaks (named A3, A2 and A1) by gel-filtration chromatography. Their estimated molecular weights were: more than 250,000, 150,000 and 17,000 respectively. 2. The purified acid form appeared as a single band of protein (Mr = 28,000) on electrophoresis in the presence of sodium dodecyl sulphate, suggesting that forms A3 and A2 are multimeric forms of beta-galactosidase A1. 3. Treatment with neuraminidase from Clostridium perfringens converts form A3 into a more basic form. This phenomenon occurs also when this form is stored for a week at 4 degrees C and parallels its disaggregation. 4. The data suggest that the sialic acids present in the multimeric forms are involved in the aggregation of the acidic form of beta-galactosidase.

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Read article at publisher's site: https://doi.org/10.1016/0020-711x(87)90081-4

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