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MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.

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Affiliations

  • 1. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
      Authors
    • Gwosch KC 1
    • Pape JK 1
    • Balzarotti F 1
    • Hell SW 1
    (4 authors)
  • 2. Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
      Authors
    • Hoess P 2
    • Ellenberg J 2
    • Ries J 2
    (3 authors)

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Nature Methods, 13 Jan 2020, 17(2):217-224
https://doi.org/10.1038/s41592-019-0688-0 PMID: 31932776 

A comment on this article appears in "Reply to: Assessment of 3D MINFLUX data for quantitative structural biology in cells." Nat Methods. 2023 Jan;20(1):52-54. A comment on this article appears in "Assessment of 3D MINFLUX data for quantitative structural biology in cells." Nat Methods. 2023 Jan;20(1):48-51. This article is based on a previously available preprint.

Abstract 

The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.

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Read article at publisher's site: https://doi.org/10.1038/s41592-019-0688-0

Read article for free, from open access legal sources, via Unpaywall: https://www.biorxiv.org/content/biorxiv/early/2019/08/13/734251.full.pdfUnpaywall

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