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Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.

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Affiliations

  • 1. Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112.
      Authors
    • Mansour SL 1
    (1 author)

Nature, 01 Nov 1988, 336(6197):348-352
https://doi.org/10.1038/336348a0 PMID: 3194019 

Abstract 

Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

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