Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesei by CRISPR/Cas9-coupled in vivo gRNA transcription.

Author information

Affiliations

  • 1. State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.
      Authors
    • Wu C 1
    • Chen Y 1
    • Qiu Y 1
    • Niu X 1
    • Chen J 1
    • Yao H 1
    • Wang W 1
    • Ma Y 1
    (8 authors)
  • 2. The First Affiliated Middle School of East China Normal University, Shanghai, 200086, China.
      Authors
    • Zhu N 2
    (1 author)

ORCIDs linked to this article

Biotechnology Letters, 16 Apr 2020, 42(7):1203-1210
https://doi.org/10.1007/s10529-020-02887-0 PMID: 32300998 

Abstract 

Objective

To simplify CRISPR/Cas9 genome editing in the industrial filamentous fungus Trichoderma reesei based on in vivo guide RNA (gRNA) transcription.

Results

Two putative RNA polymerase III U6 snRNA genes were identified in the genome of T. reesei QM6a by BLASTN using Myceliophthora. thermophila U6 snRNA gene as the template. The regions approximately 500 bp upstream of two U6 genes were efficient promoters for the in vivo expression of gRNA. The CRISPR system consisting of Cas9 and in vivo synthesized gRNA under control of the T. reesei U6 snRNA promoters was sufficient to cause a frameshift mutation in the ura5 gene via non-homologous end-joining-mediated events.

Conclusions

We report a simple gene editing method using a CRISPR/Cas9-coupled in vivo gRNA transcription system in T. reesei.

Full text links 

Read article at publisher's site: https://doi.org/10.1007/s10529-020-02887-0

Citations & impact 

Impact metrics

8
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/80059521
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/80059521

Article citations

Go to all (8) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Open Funding Project of the State Key Laboratory of Bioreactor Engineering

    Postdoctoral Research Foundation of China (1)

    the Fundamental Research Funds for the Central Universities (1)