Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Remarkably Complex Microbial Community Composition in Bromeliad Tank Waters Revealed by eDNA Metabarcoding.

Author information

Affiliations

  • 1. Laboratório de Biologia Genômica e Molecular, Escola de Ciências da Saúde e da Vida, PUCRS, Av. Ipiranga, 6681, prédio 12., Porto Alegre, Rio Grande do Sul, 90619-900, Brazil.
      Authors
    • Simão TLL 1
    • Eizirik E 1
    (2 authors)
  • 2. Laboratório de Ecologia Aquática, Escola de Ciências da Saúde e da Vida, PUCRS, Av. Ipiranga, 6681, prédio 12., Porto Alegre, Rio Grande do Sul, 90619-900, Brazil.
      Authors
    • Utz LRP 2
    (1 author)
  • 3. Department of Integrative Structural and Computational Biology, Scripps Research Translational Institute, 3344 North Torrey Pines Court, Suite 300, La Jolla, California, 92037, USA.
      Authors
    • Dias R 3
    (1 author)
  • 4. Instituto do Petróleo e Recursos Naturais, PUCRS, Av. Ipiranga 6681, prédio 96J, Porto Alegre, Rio Grande do Sul, 90619-900, Brazil.
      Authors
    • Giongo A 4
    (1 author)
  • 5. Department of Microbiology and Cell Science, University of Florida, 1052 Museum Road, P.O. Box 110700, Gainesville, Florida, 32608, USA.
      Authors
    • Triplett EW 5
    (1 author)

ORCIDs linked to this article

The Journal of Eukaryotic Microbiology, 03 Aug 2020, 67(5):593-607
https://doi.org/10.1111/jeu.12814 PMID: 32562451 

Abstract 

To investigate patterns of biotic community composition at different spatial scales and biological contexts, we used environmental DNA metabarcoding to characterize eukaryotic and prokaryotic assemblages present in the phytotelmata of three bromeliad species (Aechmea gamosepala, Vriesea friburgensis, and Vriesea platynema) at a single Atlantic Forest site in southern Brazil. We sampled multiple individuals per species and multiple tanks from each individual, totalizing 30 samples. We observed very high levels of diversity in these communities, and remarkable variation across individuals and even among tanks from the same individual. The alpha diversity was higher for prokaryotes than eukaryotes, especially for A. gamosepala and V. platynema samples. Some biotic components appeared to be species-specific, while most of the biota was shared among species, but varied substantially in frequency among samples. Interestingly, V. friburgensis communities (which were sampled at nearby locations) tended to be more heterogeneous across samples, for both eukaryotes and prokaryotes. The opposite was true for V. platynema, whose samples were more broadly spaced but whose communities were more similar to each other. Our results indicate that additional attention should be devoted to within-individual heterogeneity when assessing bromeliad phytotelmata biodiversity, and highlight the complexity of the biotic assemblages gathered in these unique habitats.

Full text links 

Read article at publisher's site: https://doi.org/10.1111/jeu.12814

Read article for free, from open access legal sources, via Unpaywall: https://repositorio.pucrs.br/dspace/bitstream/10923/18439/2/Remarkably_Complex_Microbial_Community_Composition_in_Bromeliad_Tank_Waters_Revealed_by_eDNA_Metabarcoding.pdfUnpaywall

Citations & impact 

Impact metrics

3
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/85098414
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/85098414

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1111/jeu.12814

Supporting
Mentioning
Contrasting
2
4
0

Article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Conselho Nacional de Desenvolvimento Científico e Tecnológico

    Coordenação de Aperfeiçoamento de Pessoal de Nível Superior