Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Complete chloroplast genomes of two medicinal Swertia species: the comparative evolutionary analysis of Swertia genus in the Gentianaceae family.

Author information

Affiliations

  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, No. 151, Malianwa North Road, Hai Dian District, Beijing, 100193, People's Republic of China.
      Authors
    • Du Q 1
    • Li J 1, 4
    • Chen H 1
    • Jiang M 1
    • Chen Z 1, 4
    • Liu C 1
    (6 authors)
  • 2. College of Pharmacy, Heze University, No.2269, University Road, Mudan District, Heze City, Shandong Province, 274015, People's Republic of China.
      Authors
    • Wang L 2
    (1 author)
  • 3. Genepioneer Biotechnologies Inc, No. 9, Weidi Road, Qixia District, Nanjing City, Jiangsu Province, 210000, People's Republic of China.
      Authors
    • Jiang C 3
    • Gao H 3
    (2 authors)
  • 4. Xiangnan University, No. 889, Chenzhou dadao, Chenzhou City, Hunan Province, 423000, People's Republic of China.
      Authors
    • Li J 1, 4
    • Chen Z 1, 4
    • Wang B 4
    (3 authors)

ORCIDs linked to this article

Show all (10)

Planta, 09 Sep 2022, 256(4):73
https://doi.org/10.1007/s00425-022-03987-z PMID: 36083348 

Abstract 

Main conclusion

The complete chloroplast genome of Swertia kouitchensis has been sequenced and assembled, compared with that of S. bimaculata to determine the evolutionary relationships among species of the Swertia in the Gentianaceae family. Swertia kouitchensis and S. bimaculata are from the Gentianaceae family. The complete chloroplast genome of S. kouitchensis was newly assembled, annotated, and analyzed by Illumina Hiseq 2500 platform. The chloroplast genomes of the two species encoded a total of 133, 134 genes, which included 88-89 protein-coding genes, 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA genes. One intron was contained in each of the eight protein-coding genes and eight tRNA-coding genes, whereas two introns were found in two genes (ycf3 and clpP). The most abundant codon of the two species was for isoleucine, and the least abundant codon was for cysteine. The number of microsatellite repeat sequences was twenty-eight and thirty-two identified in the chloroplast genomes of S. kouitchensis and S. bimaculata, respectively. A total of 1127 repeat sequences were identified in all the 23 Swertia chloroplast genomes, and they fell into four categories. Furthermore, five divergence hotspot regions can be applied to discriminate these 23 Swertia species through genomes comparison. One pair of genus-specific DNA barcodes primer has been accurately identified. Therefore, the diverse regions cloned by a specific primer may become an effective and powerful molecular marker for the identification of Swertia genus. Moreover, four genes (ccsA, ndhK, rpoC1, and rps12) were positive selective pressure. The phylogenetic tree showed that the 23 Swertia species were clustered into a large clade including four evident subbranches, whereas the two species of S. kouitchensis and S. bimaculata were separately clustered into the diverse but correlated species group.

Full text links 

Read article at publisher's site: https://doi.org/10.1007/s00425-022-03987-z

References 

Articles referenced by this article (59)

Show 10 more references (10 of 59)

Citations & impact 

Impact metrics

5
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/135863750
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/135863750

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1007/s00425-022-03987-z

Supporting
Mentioning
Contrasting
0
5
1

Article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Chinese Academy of Medical Sciences, Innovation Funds for Medical Sciences (1)

Hunan technological innovation guidance project (1)

National Science &Technology Fundamental Resources Investigation Program of China (1)

National Science &Technology Fundamental Resources Investigation Program of China (1)

National Science Foundation (1)

Qinghai Provincial Key Laboratory of Phytochemistry of Qinghai Tibet Plateau (1)