Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Structure and Functional Properties of Bacterial Communities in Surface Sediments of the Recently Declared Nutrient-Saturated Lake Villarrica in Southern Chile.

Author information

Affiliations

  • 1. Laboratorio de Ecología Microbiana Aplicada (EMALAB), Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145, Temuco, Chile.
      Authors
    • Campos MA 1
    • Acuña JJ 1
    • Rilling JI 1
    • Ruiz T 1
    • Carrazana E 1
    • Reyno C 1
    • Jorquera MA 1
    (7 authors)
  • 2. The BioTechnology Institute, University of Minnesota, 140 Gortner Lab, 1479 Gortner Ave., St Paul, MN, 55108-6106, USA.
      Authors
    • Zhang Q 2
    • Elias M 2
    • Sadowsky MJ 2
    (3 authors)
  • 3. Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.
      Authors
    • Hollenback A 3
    • Gray K 3
    • Jaisi DP 3
    (3 authors)
  • 4. Soil and Water Sciences Department, University of Florida, PO Box 110290, Gainesville, FL, 32608-32611, USA.
      Authors
    • Ogram A 4
    (1 author)
  • 5. State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China.
      Authors
    • Bai J 5
    • Zhang L 5
    (2 authors)
    • Show all (7)

ORCIDs linked to this article

Microbial Ecology, 08 Feb 2023, 86(3):1513-1533
https://doi.org/10.1007/s00248-023-02173-2 PMID: 36752910 

Abstract 

Lake Villarrica, one of Chile's main freshwater water bodies, was recently declared a nutrient-saturated lake due to increased phosphorus (P) and nitrogen (N) levels. Although a decontamination plan based on environmental parameters is being established, it does not consider microbial parameters. Here, we conducted high-throughput DNA sequencing and quantitative polymerase chain reaction (qPCR) analyses to reveal the structure and functional properties of bacterial communities in surface sediments collected from sites with contrasting anthropogenic pressures in Lake Villarrica. Alpha diversity revealed an elevated bacterial richness and diversity in the more anthropogenized sediments. The phylum Proteobacteria, Bacteroidetes, Acidobacteria, and Actinobacteria dominated the community. The principal coordinate analysis (PCoA) and redundancy analysis (RDA) showed significant differences in bacterial communities of sampling sites. Predicted functional analysis showed that N cycling functions (e.g., nitrification and denitrification) were significant. The microbial co-occurrence networks analysis suggested Chitinophagaceae, Caldilineaceae, Planctomycetaceae, and Phycisphaerae families as keystone taxa. Bacterial functional genes related to P (phoC, phoD, and phoX) and N (nifH and nosZ) cycling were detected in all samples by qPCR. In addition, an RDA related to N and P cycling revealed that physicochemical properties and functional genes were positively correlated with several nitrite-oxidizing, ammonia-oxidizing, and N-fixing bacterial genera. Finally, denitrifying gene (nosZ) was the most significant factor influencing the topological characteristics of co-occurrence networks and bacterial interactions. Our results represent one of a few approaches to elucidate the structure and role of bacterial communities in Chilean lake sediments, which might be helpful in conservation and decontamination plans.

Full text links 

Read article at publisher's site: https://doi.org/10.1007/s00248-023-02173-2

References 

Articles referenced by this article (39)

Show 10 more references (10 of 39)

Citations & impact 

This article has not been cited yet.

Impact metrics

Alternative metrics

Altmetric item for https://www.altmetric.com/details/142796398
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/142796398

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Agencia Nacional de Investigación y Desarrollo (2)