Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples.

Author information

Affiliations

  • 1. Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara; Center on Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara;
      Authors
    • Plebani R 1
    • Romano M 1
    (2 authors)
  • 2. Center on Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara.
      Authors
    • D'Alessandro A 2
    (1 author)
  • 3. Center on Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara; Department of Medicine and Aging Sciences, University "G. d'Annunzio" of Chieti-Pescara.
      Authors
    • Lanuti P 3
    • Simeone P 3
    • Marchisio M 3
    (3 authors)
  • 4. Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara.
      Authors
    • Cinalli M 4
    • Cappabianca F 4
    • Mucilli F 4
    (3 authors)
  • 5. U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico di Milano.
      Authors
    • Righi I 5
    (1 author)
    • Show all (8)

ORCIDs linked to this article

Journal of Visualized Experiments : Jove, 03 Feb 2023, (192)
https://doi.org/10.3791/64885 PMID: 36808144 

Abstract 

The availability of cells isolated from healthy and diseased tissues and organs represents a key element for personalized medicine approaches. Although biobanks can provide a wide collection of primary and immortalized cells for biomedical research, these do not cover all experimental needs, particularly those related to specific diseases or genotypes. Vascular endothelial cells (ECs) are key components of the immune inflammatory reaction and, thus, play a central role in the pathogenesis of a variety of disorders. Notably, ECs from different sites display different biochemical and functional properties, making the availability of specific EC types (i.e., macrovascular, microvascular, arterial, and venous) essential for designing reliable experiments. Here, simple procedures to obtain high-yield, virtually pure human macrovascular and microvascular endothelial cells from the pulmonary artery and lung parenchyma are illustrated in detail. This methodology can be easily reproduced at a relatively low cost by any laboratory to achieve independence from commercial sources and obtain EC phenotypes/genotypes that are not yet available.

Full text links 

Read article at publisher's site: https://doi.org/10.3791/64885

Citations & impact 

This article has not been cited yet.

Impact metrics

Alternative metrics

Altmetric item for https://www.altmetric.com/details/164591293
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/164591293

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.