The melon (Cucumis melo L.), a fruit crop of significant economic importance, is prized for its sweet and succulent fruits. Among variations of soluble sugars, sucrose, a disaccharide composed of glucose and fructose, is a key carbohydrate present in melon fruits. The sucrose content also determines the quality and value of melon fruits. However, the accumulation of sucrose is a complex process involving the coordinated actions of multiple enzymes and pathways. In melon species, there are two types of fruit ripening modes including climacteric and non-climacteric. Due to this biological characteristic, melon is emerging as a good model for studying the ripening process. Ethylene is a well-known phytohormone regulating the ripening of climacteric fruits. Recently, a few studies have elucidated a primary ethylene-dependent signaling pathway of sucrose accumulation in melon fruits. This review aims to provide a careful overview of the sucrose biosynthesis pathways in melon. It is essential to understand the molecular mechanisms of sucrose metabolism as well as its regulation mode. The information will be useful for developing molecular marker-assisted breeding as well as genetic engineering strategies aiming to improve the sucrose content and quality of melon fruits. In addition, even though limited, the impacts of genetic background and environmental factors on sucrose accumulation in melon fruits are also discussed. These are useful for practical applications in melon cultivation and quality management.