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Identification of differentially expressed genes and metabolism signaling pathway in the spleen of broilers supplemented with probiotic Bacillus spp.

Author information

Affiliations

  • 1. Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, Viet Nam.
      Authors
    • Truong AD 1
    • Tran HTT 1
    • Thi Nguyen H 1
    • Thi Chu N 1
    • Phan L 1
    • Thi Phan H 1
    • Thi Pham N 1
    • Vu Dang H 1
    (8 authors)
  • 2. Department of Food Engineering, School of Chemistry and Life Sciences, Hanoi University of Science and Technology (HUST), Hanoi 100000, Viet Nam.
      Authors
    • Nguyen VH 2
    • Ho PH 2
    (2 authors)
  • 3. Department of Bioengineering, School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Viet Nam.
      Authors
    • Nguyen LH 3
    (1 author)
  • 4. Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi 100000, Viet Nam; Department of Livestock Production, Ministry of Agriculture and Rural Development, 16 Thuy Khue, Tay Ho, Hanoi 100000, Viet Nam.
      Authors
    • Pham DK 4
    (1 author)

ORCIDs linked to this article

Veterinary Immunology and Immunopathology, 18 Apr 2024, 272:110755
https://doi.org/10.1016/j.vetimm.2024.110755 PMID: 38643554 

Abstract 

Probiotics are essential in the body's nutrients, improving the ratio of meat to meat, immune response, and preventing diseases. In this study, RNA-sequencing (RNA-seq) was used to identify the differentially expressed genes (DEGs), enriched related pathways, and Gene Ontology (GO) terms among blank negative control (NC), supplemented with Bacillus spp. (BS) and commercial probiotic (PC) groups after a 42-day fed supplementation. The results showed that 2005, 1356, and 2189 DEGs were significantly altered in BS vs. NC, PC vs NC, and BS vs PC groups, respectively. On the other hand, 9 DEGs were further validated by qRT-PCR, indicating that the qRT-PCR and RNA-Seq results were more consistent. Therefore, the GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs showed that the DEGs were mainly enriched to metabolism signalling pathways (alpha-linolenic acid metabolism, linoleic acid metabolism, tryptophan metabolism, tyrosine metabolism, ether lipid metabolism, and metabolic pathway, etc) and immune response pathways (cytokine-cytokine receptor interaction, MAPK signalling pathway, and intestinal immune network for IgA production, neuroactive ligand-receptor interaction etc). These results will provide a better understanding of the role of probiotics in chicken development and provide basic information on the genetic development of chickens.

Full text links 

Read article at publisher's site: https://doi.org/10.1016/j.vetimm.2024.110755

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Funding 

Funders who supported this work.

Ministry of Science and Technology (1)