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Unraveling hallmark suitability for staging pre- and post-implantation stem cell models.

Author information

Affiliations

  • 1. Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France.
      Authors
    • Onfray C 1
    • Chevolleau S 1
    • Moinard E 1
    • Girard O 1
    (4 authors)
  • 2. Université Paris Cité, CNRS, Epigenetics and Cell Fate, 75013 Paris, France.
      Authors
    • Mahadik K 2
    • Ouimette JF 2
    • Rougeulle C 2
    (3 authors)
  • 3. KU Leuven - University of Leuven, Department of Development and Regeneration, Leuven Institute for Single Cell Omics and Leuven Stem Cell Institute, Herestraat 49, 3000 Leuven, Belgium.
      Authors
    • Allsop R 3
    • Georgolopoulos G 3
    • Pasque V 3
    (3 authors)
  • 4. University Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, 35000 Rennes, France; University Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, Protim Core Facility, 35000 Rennes, France.
      Authors
    • Lavigne R 4
    • Pineau C 4
    (2 authors)
  • 5. Nantes Université, CNRS, Inserm, CRCI2NA, 44000 Nantes, France.
      Authors
    • Renoult O 5
    • Pecqueur C 5
    (2 authors)
    • Show all (9)

ORCIDs linked to this article

Cell Reports, 17 May 2024, 43(5):114232
https://doi.org/10.1016/j.celrep.2024.114232 PMID: 38761378 

Abstract 

The advent of novel 2D and 3D models for human development, including trophoblast stem cells and blastoids, has expanded opportunities for investigating early developmental events, gradually illuminating the enigmatic realm of human development. While these innovations have ushered in new prospects, it has become essential to establish well-defined benchmarks for the cell sources of these models. We aimed to propose a comprehensive characterization of pluripotent and trophoblastic stem cell models by employing a combination of transcriptomic, proteomic, epigenetic, and metabolic approaches. Our findings reveal that extended pluripotent stem cells share many characteristics with primed pluripotent stem cells, with the exception of metabolic activity. Furthermore, our research demonstrates that DNA hypomethylation and high metabolic activity define trophoblast stem cells. These results underscore the necessity of considering multiple hallmarks of pluripotency rather than relying on a single criterion. Multiplying hallmarks alleviate stage-matching bias.

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Read article at publisher's site: https://doi.org/10.1016/j.celrep.2024.114232

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