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Copaifera spp. oleoresins control Trypanosoma cruzi infection in human trophoblast cells (BeWo) and placental explants.

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Author information

Affiliations

  • 1. Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
      Authors
    • Júnior JPL 1
    • Teixeira SC 1
    • de Souza G 1
    • Faria GV 1
    • Almeida MPO 1
    • Luz LC 1
    • Paschoalino M 1
    • Dos Santos NCL 1
    • de Oliveira RM 1
    • Martínez AFF 1
    • Rosini AM 1
    • Ferro EAV 1
    • Barbosa BF 1
    (13 authors)
  • 2. Department of Parasitology, Institute of Biomedical Sciences, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
      Authors
    • Franco PS 2
    (1 author)
  • 3. Nucleus of Research in Technological and Exact Sciences, Universidade de Franca, Franca, SP, Brazil.
      Authors
    • Ambrosio MALV 3
    • Veneziani RCS 3
    (2 authors)
  • 4. School of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
      Authors
    • Bastos JK 4
    (1 author)
  • 5. Institute of Natural and Biological Sciences, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
      Authors
    • Gomes AO 5
    (1 author)
    • Show all (8)

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 11 Sep 2024, 179:117425
https://doi.org/10.1016/j.biopha.2024.117425 PMID: 39265235 

Abstract 

Congenital Chagas disease (CCD) is a worldwide neglected problem with significant treatment limitations. This study aimed to evaluate the potential of Copaifera spp. oleoresins (ORs) against Trypanosoma cruzi infection in trophoblast cells (BeWo lineage) and human chorionic villous explants (HCVE). The cytotoxicity of ORs was investigated using LDH and MTT assays. T. cruzi (Y strain) proliferation, invasion and reversibility were assessed in OR-treated BeWo cells, and proliferation was evaluated in OR-treated HCVE. The ultrastructure of T. cruzi trypomastigotes and amastigotes treated with ORs were analyzed by scanning and transmission electronic microscopy. ROS production in infected and treated BeWo cells and cytokines in BeWo and HCVE were measured. The ORs irreversibly decreased T. cruzi invasion, proliferation and release in BeWo cells by up to 70 %, 82 % and 80 %, respectively, and reduced parasite load in HCVE by up to 80 %. Significant structural changes in treated parasites were observed. ORs showed antioxidant capacity in BeWo cells, reducing ROS production induced by T. cruzi infection. Also, T. cruzi infection modulated the cytokine profile in both BeWo cells and HCVE; however, treatment with ORs upregulated cytokines decreased by T. cruzi infection in BeWo cells, while downregulated cytokines increased by the T. cruzi infection in HCVE. In conclusion, non-cytotoxic concentrations of Copaifera ORs demonstrated promising potential for controlling T. cruzi infection in models of the human maternal-fetal interface.

Full text links 

Read article at publisher's site: https://doi.org/10.1016/j.biopha.2024.117425

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Funding 

Funders who supported this work.

Coordination of Higher Education Personnel Improvement

    Minas Gerais State Foundation of Support to the Research

      National Council for Scientific and Technological Development

        Universidade do Estado de Minas Gerais (1)