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Toxic metals in Amazonian soil modify the bacterial community associated with Diplopoda.

Author information

Affiliations

  • 1. Laboratory of Biotechnology and Microbial Ecology (LABEM), Department of Biosciences, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa, 2367, 78060-900 Cuiabá, Mato Grosso, Brazil.
      Authors
    • Santos-Silva L 1
    • Roque WF 1
    • Mello IS 1
    • Soares MA 1
    (4 authors)
  • 2. Laboratory of Biotechnology and Microbial Ecology (LABEM), Department of Biosciences, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa, 2367, 78060-900 Cuiabá, Mato Grosso, Brazil; Federal Institute of Education, Science and Technology of Mato Grosso (IFMT), Av. Ver. Juliano da Costa Marques, S/N - Bela Vista, 78050-560 Cuiabá, Mato Grosso, Brazil.
      Authors
    • de Moura JM 2
    (1 author)
  • 3. Bioinformatics Laboratory, Department of Agricultural, Livestock and Environmental Biotechnology, São Paulo State University (UNESP), School of Agricultural and Veterinary Sciences, Jaboticabal, S/N - Vila Industrial, 14884-900 São Paulo, Brazil.
      Authors
    • de Carvalho LAL 3
    • Pinheiro DG 3
    (2 authors)
  • 4. Laboratory of Zoological Collections (LCZ), Butantã, Av. Vital Brazil, 1500, 05503-900 São Paulo, Brazil.
      Authors
    • Bouzan RS 4
    • Brescovit AD 4
    (2 authors)
  • 5. Interdisciplinary Chemical Research Laboratory (LIPEQ), Institute of Natural, Human and Social Sciences, Federal University of Mato Grosso, Av. das Figueiras, 6.669, Aquarela das Artes, Sinop, Mato Grosso, Brazil.
      Authors
    • de Andrade RLT 5
    (1 author)
    • Show all (7)

The Science of the Total Environment, 16 Oct 2024, 955:176915
https://doi.org/10.1016/j.scitotenv.2024.176915 PMID: 39419211 

Abstract 

Toxic metal pollution in the Amazon is a serious problem that reduces the quality of water, soil, air, and consequently alters communities of fauna, flora, and microbiota, harming human health and well-being. Our aim was to determine the impact of toxic metals on the structure of the bacterial community associated with Diplopoda in the Amazon rainforest. Animals were kept in microcosms contaminated with cadmium (50 mg.kg-1), mercury (35 mg.kg-1) and no toxic metal (control). The intestinal and molting chamber microbiota were accessed by culture-dependent and culture-independent methods (16S metabarcoding). The cultivated strains were identified, and their functional traits evaluated: secretion of enzymes, growth at different pH, resistance to metals and antibiotics, and ability to reduce toxic effects of metals on plants. Our research described Brachyurodesmus albus, a new species of Diplopoda. We obtained 177 isolates distributed in 35 genera and 61 species of bacteria (Pseudomonadota, Bacillota, Bacteroidota and Actinomycetota) associated with the gut and molting chamber of B. albus. Metabarcoding data provided a more robust access to the bacterial community, resulting in 24 phyla, 561 genera and 6792 ASVs. The presence of metal Cd and Hg alters the composition and abundance of bacteria associated with B. albus (PERMANOVA p < 0.05). The microhabitat (gut and molting chamber) harbours bacterial communities that differ in composition and abundance (PERMANOVA p < 0.05). The presence of Cd and Hg alters important metabolic pathways related to the prokaryotic defense system; antimicrobial resistance genes, endocytosis and secretion system, estimated by PICRUSt. Bacteria selected with high resistance to Cd and Hg buffer the toxic effect of metals on tomato seedlings. This work describes B. albus and concludes that its diverse bacterial microbiota is altered by soil contamination by toxic metals, as well as being an important repository for prospecting strains to be applied in bioremediation programs.

Full text links 

Read article at publisher's site: https://doi.org/10.1016/j.scitotenv.2024.176915

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Funding 

Funders who supported this work.

Coordination of Higher Education Personnel Improvement

    Foundation for Research Support of Amazonas State

      Foundation for Research Support of Mato Grosso State

        National Council for Scientific and Technological Development