Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides.

1. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
Authors
Sun G¹
Xia Y¹
Zhu Q¹
Ding F¹
Zhang Z¹
Li X¹
Mi X¹
Chen J¹
Yao R¹
Zhang S¹
Ouyang H¹
Chen X¹
Liu T¹
Jiang H¹
Zhao Y¹
Qiu M¹
Ye W¹
Duan K¹
Ma Z¹
Dong S¹
Wang Y¹
Wang Y¹
(22 authors)
2. Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Technology Innovation Center for Green Agriculture, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Authors
Li K²
Gu H²
Yin H²
(3 authors)

Science China. Life Sciences, 13 Nov 2024,
https://doi.org/10.1007/s11427-024-2724-5 PMID: 39549112

Abstract

Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity. Herein, we identified a polysaccharide lyase 1 protein, PsPL1, which acts as an essential virulence factor of P. sojae infection in soybean. However, the overexpression of PsPL1 in P. sojae reduced infection and triggered enhanced immune responses in soybean. PsPL1 exhibited pectin lyase activity and degraded plant pectin to generate pectin oligosaccharides (POSs) with a polymerization degree of 3-14, exhibiting different levels of acetylation and methylation modifications. PsPL1 and the degraded pectin products triggered immune responses in soybean and different Solanaceous plants. The PsPL1-triggered immune responses required RSPL1, a membrane-localized leucine-rich repeat receptor-like protein, which is essential for Phytophthora resistance. Conversely, the PsPL1-degraded product-triggered immune responses depended on the membrane-localized lysin motif receptor-like kinase CERK1. This study reveals that the pectin lyase exhibits a dual immunogenic role during P. sojae infection, which activates plant resistance through different immune receptors and provides novel insights into the function of pectin lyase in host-pathogen interactions.

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References

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Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides.

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Authors

Abstract

Full text links

References

Surface Sensor Systems in Plant Immunity.

We be jammin': an update on pectin biosynthesis, trafficking and dynamics.

BAK1 is required for the attenuation of ethylene-inducing xylanase (Eix)-induced defense responses by the decoy receptor LeEix1.

Pectate lyase affects pathogenicity in natural isolates of Colletotrichum coccodes and in pelA gene-disrupted and gene-overexpressing mutant lines.

Improved prediction of signal peptides: SignalP 3.0.

Bioinformatic characterisation of genes encoding cell wall degrading enzymes in the Phytophthora parasitica genome.

Function, Discovery, and Exploitation of Plant Pattern Recognition Receptors for Broad-Spectrum Disease Resistance.

A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides.

Homeostasis of cell wall integrity pathway phosphorylation is required for the growth and pathogenicity of Magnaporthe oryzae.

Convergent evolution of immune receptors underpins distinct elicitin recognition in closely related Solanaceous plants.

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Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides.

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