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Aloperine Attenuates UVB-induced Damage in Skin Fibroblasts Via Activating TFE3/Beclin-1-Mediated Autophagy.

Author information

Affiliations

  • 1. Laboratory of Urology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, Guangdong, China.
      Authors
    • Qiu M 1
    • Li J 1
    • Zhang S 1
    (3 authors)
  • 2. Experimental Animal Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, Guangdong, China.
      Authors
    • Liang J 2
    • Wang X 2
    • Liu J 2
    (3 authors)

ORCIDs linked to this article

Protein and Peptide Letters, 15 Nov 2024,
https://doi.org/10.2174/0109298665335370241017055831 PMID: 39558499 

Abstract 

Background

Aloperine (ALO) is an important active ingredient in the traditional Chinese medicinal plant Sophora alopecuroides L and has a significant autophagy-stimulating effect. The effect of ALO on cytotoxicity caused by UVB radiation in skin fibroblasts and the potential mechanism remains unclear.

Objective

The present study aimed to assess the effect of ALO on UVB-induced damage in skin fibroblasts and investigate its possible mechanism.

Methods

Cell viability, cytotoxicity, caspase-Glo 3/7 activity, apoptosis, and protein expression were measured in UVB-treated skin fibroblasts in the presence or absence of ALO. Autophagy inhibitors (chloroquine and bafilomycin A1) and TFE3 siRNA transfection were used to elucidate the potential mechanisms further.

Results

These data demonstrate that ALO attenuated cell viability inhibition, apoptosis, cytotoxicity, and alterations in autophagy-related proteins caused by UVB exposure in skin fibroblasts. ALO stimulates autophagy activation and TFE3 nuclear localization in UVB-treated skin fibroblasts. Furthermore, treatment with autophagy inhibitors and TFE3 siRNA reversed the effects of ALO on UVB-treated skin fibroblasts.

Conclusion

These results suggest that ALO protects skin fibroblasts against UVB-induced cytotoxicity by stimulating TFE3/Beclin-1-mediated autophagy.

Full text links 

Read article at publisher's site: https://doi.org/10.2174/0109298665335370241017055831