Preparation of viable astrocytes from the developing cerebellum.

Cohen J; Woodhams PL; Balázs R

doi:10.1016/0006-8993(79)90679-6

Abstract

A cell fraction enriched in viable astrocytes has been prepared from the developing rat cerebellum. The isolated astrocytes were identified by indirect immunofluorescence using antiserum to glial fibrillary acidic protein (GFAP). In comparison with the low incidence (6%) of GFAP-positive cells in the unfractionated cell suspension from trypsinized cerebellar tissue, these accounted for half of the cells in the final preparation. This was achieved by treatment at 6 days of age with hydroxyurea (an inhibitor of DNA synthesis) which enriched the tissue in GFAP-positive astrocytes at day 8. At the same time, by selectively removing the heterogeneously sized replicating cells, hydroxyurea treatment allowed resolution of a fraction enriched in GFAP-positive cells using unit gravity sedimentation. The isolated astrocytes differentiated in culture producing, by 3 days in vitro, a dense network of fine GFAP-containing processes.

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References

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Preparation of viable astrocytes from the developing cerebellum.

Abstract

Full text links

References

DNA metabolism and cell proliferation

Isolation and biochemical characterisation of morphologically defined structures, including cell types from the cerebellum

Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence.

Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein.

Astrocyte-specific protein and radial glia in the cerebral cortex of newborn rat.

Specificity of the glial fibrillary acidic protein for astroglia.

Protein turnover in cell-enriched fractions from rabbit brain.

Glial fibrillary acidic protein in primary astroglial cell cultures derived from newborn rat brain.

Demonstration of a protein immunochemically related to glial fibrillary acidic protein in human fibroblasts in culture.

Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.

Citations & impact

Impact metrics

Citations of article over time

Smart citations by scite.ai
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1016/0006-8993(79)90679-6

Article citations

Effects of estrogen receptor agonists on regulation of the inflammatory response in astrocytes from young adult and middle-aged female rats.

An in vitro model for toxicological investigations of environmental neurotoxins in primary neuronal cell cultures.

Cellular origins of cyclic GMP responses to excitatory amino acid receptor agonists in rat cerebellum in vitro.

Gradient isolation of glial cells: evidence that flat epithelial cells are astroglial cell precursors.

Beta-adrenergic receptors of cerebellar astrocytes in culture: intact cells versus membrane preparation.

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