Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.

Williams DA; Lemischka IR; Nathan DG; Mulligan RC

doi:10.1038/310476a0

Abstract

An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme.

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References

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Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.

Abstract

Full text links

References

The regulated expression of beta-globin genes introduced into mouse erythroleukemia cells.

Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.

A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.

Glucocorticoid regulation of the Ha-MuSV p21 gene conferred by sequences from mouse mammary tumor virus.

Metallothionein-human GH fusion genes stimulate growth of mice.

Cellular interactions in haematopoiesis.

Nathan, D. G. & Oski, F. A. (eds) Hematology of Infancy and Childhood (Saunders, Philadelphia, 1981).

Antigenically distinct subpopulations of myeloid progenitor cells (CFU-GM) in human peripheral blood and marrow.

Colony formation in agar by adult bone marrow multipotential hemopoietic cells.

Improved plasma culture system for production of erythrocytic colonies in vitro: quantitative assay method for CFU-E.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Smart citations by scite.ai
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1038/310476a0

Article citations

International Society for Cell & Gene Therapy Stem Cell Engineering Committee report on the current state of hematopoietic stem and progenitor cell-based genomic therapies and the challenges faced.

Gene Therapy for β-Hemoglobinopathies: From Discovery to Clinical Trials.

Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years.

Gene therapy for inborn errors of immunity: past, present and future.

The long road traveled in hematopoietic stem cell gene therapy.

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