Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.

Accolla RS; Sekaly RP; McDonald AP; Corte G; Gross N; Carrel S

doi:10.1002/eji.1830120212

Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.

European Journal of Immunology, 01 Feb 1982, 12(2):166-169
https://doi.org/10.1002/eji.1830120212 PMID: 6176457

Abstract

The expression at the cell surface level of two previously defined human Ia antigen subsets, NG1 and NG2, was studied. Two monoclonal hybridoma antibodies, D1-12 (anti-NG1) and D4-22 (anti-NG2), labeled with distinct fluorochromes, were used as probes. The results indicate that NG1 and NG2 molecules are simultaneously expressed on the same cell, and moreover, that they are identifiable as spatially separate structures. In addition, when a series of 6 other anti-Ia monoclonal antibodies previously described were analyzed in a checkerboard test of binding inhibition at the cell surface level, they were found to closely mimic the reactivity pattern of either D1-12 or D4-22 antibody, suggesting that NG1 and NG2 Ia subsets express independent clusters of highly immunogenic epitopes.

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References

Articles referenced by this article (12)

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Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.

Abstract

Full text links

References

Title not supplied

Orientation of cell-surface antigens in the lipid bilayer of lymphocyte plasma membrane.

Immunological dissection of human Ia molecules.

Molecular identification of human Ia antigens coded for by a gene locus closely linked to HLA-DR locus.

Title not supplied

Subsets of human Ia-like molecules defined by monoclonal antibodies.

Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.

, and , Protides of Biological Fluids 1981. vol. 29, in press.

, and , in and (Eds.), Melanoma Antigens and Antibodies, Raven Press, New York 1981, in press.

Analysis of HLA-DR polymorphism by two-dimensional peptide mapping.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Article citations

An Epstein-Barr virus-associated superantigen.

T-cell-mediated epineurial vasculitis and humoral-mediated microangiopathy in cryoglobulinemic neuropathy.

TCR alpha/beta and TCR gamma/delta CD4-/CD8- HLA-DR alloreactive CTL clones do not use Fas/Fas ligand pathway to lyse their specific target cells.

Role of HIV in the pathogenesis of distal symmetrical peripheral neuropathy.

The role of HLA-DP beta residue 69 in the definition of antibody-binding epitopes.

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